Coding

Part:BBa_K1391134:Design

Designed by: Gary Burnett   Group: iGEM14_MIT   (2014-10-19)


miRNA 125b target site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequences had to be design as single strands which then would have to be annealed together.


Source

It was synthesized as the reverse complement of the actual sequence for miR-125b

References